Presentación

[ES] Presentación del pimer número de la revista Ciencia, Técnica y Mainstreaming Social (CITECMA).Puchades Pla, R. (2017). Presentación. https://doi.org/10.4995/citecma.2017.7621OJ

Os roedores das ilhas Flores e Corvo : distribuição, fertilidade e morfometria

XIII Expedição Científica do Departamento de Biologia - Flores e Corvo 2007.O conhecimento da biologia e ecologia das espécies de roedores dos Açores é escasso. Integrado na XIII Expedição Científica do Departamento de Biologia da Universidade dos Açores, realizámos uma amostragem de roedores em três habitats distintos (pastagem, floresta e lixeira) na ilha das Flores, durante três noites, e num habitat (lixeira) na ilha do Corvo, durante uma noite. A espécie Mus musculus foi capturada nos três habitats, Rattus rattus foi capturada na floresta e na pastagem e Rattus norvegicus só foi capturada na lixeira. Na ilha do Corvo não conseguimos efectuar nenhuma captura de animais destas espécies. Apresentamos os dados da fertilidade potencial das fêmeas prenhas capturados e dados sobres as medidas de algumas características da morfologia externa. Sugerimos um padrão de distribuição das três espécies para o Arquipélago dos Açores e apresentamos uma forma simples e expedita para a identificação das espécies de Rodentia nos Açores

Presentación

[ES] Presentación del tercer número de la revista Ciencia, Técnica y Mainstreaming Social (CITECMA).Puchades Pla, R. (2019). Presentación. Ciencia, Técnica y Mainstreaming Social. (3). https://doi.org/10.4995/citecma.2019.11474SWORD

Presentación

[ES] Presentación del segundo número de la revista Ciencia, Técnica y Mainstreaming Social (CITECMA).Puchades Pla, R. (2018). Presentación. https://doi.org/10.4995/citecma.2018.9858OJ

Fluor-thiol Photocoupling Reaction for Developing High Performance Nucleic Acid (NA) Microarrays

Spatially controlled anchoring of NA probes onto microscope glass slides by a novel fluor-thiol coupling reaction is performed. By this UV-initiated reaction, covalent immobilization in very short times (30 s at 254 nm) is achieved with probe densities of up to 39.6 pmol/cm2. Modulating the surface hydrophobicity by combining a hydrophobic silane and a hydrophilic silane allows the fabrication of tuned surfaces where the analyte approaches only the anchored probe, which notably reduces nonspecific adsorption and the background. The generated substrates have proven clear advantages for discriminating single-base-pair mismatches, and for detecting bacterial PCR products. The hybridization sensitivity achieved by these high-performance surfaces is about 1.7 pM. Finally, this anchoring reaction is demonstrated using two additional surfaces: polytetrafluoroethylene (PTFE) and polyvinylidene fluoride (PVDF) membranes. This provides a very interesting pathway for anchoring thiolated biomolecules onto surfaces with C–F motifs via a quick clean UV reaction.Financial support from INTERBOINTER (project CTQ2013-45875-R) and BIHOLOG (Project CTQ2016-75749-R), FEDER, and GVA PROMETEO II 2014/040 is acknowledged. P.J.-M. acknowledges the Spanish Ministry of Economy, Industry and Competitiveness for the public FPI grant (Project CTQ2013-45875-R) and the cofinancing by the European Social Fund

Primer design for SNP genotyping based on allele-specific amplification Application to organ transplantation pharmacogenomics

[EN] Diagnostic methods based on single nucleotide polymorphism (SNP) biomarkers are essential for the real adoption of personalized medicine. Allele specific amplification in a homogeneous format or combined to microarray hybridization are powerful approaches for SNP genotyping. However, primers must be properly selected to minimize cross-reactivity, dimer formation and nonspecific hybridization. This study presents a design workflow diagram for the selection of required oligonucleotides for multiplex assays. Based on thermodynamic restrictions, the oligonucleotide sets are chosen for a specific amplification of wild- and mutant-type templates. Design constraints include the structural stability of primer-template duplexes, template-probe duplexes and self-annealing complexes or hairpins for each targeted gene. The performance of the design algorithm was evaluated for the simultaneous genotyping of three SNPs related to immunosuppressive drugs administered after solid organ transplantation. The assayed polymorphisms were rs1045642 (ABCS] gene), rs1801133 (MTHFR gene) and rs776746 (CYP3A5 gene). Candidates were confirmed by discriminating homozygote and heterozygote populations using a fluorescence solution method and two colorimetric microarray methods on polycarbonate chips. The analysis of patient samples provided excellent genotyping results compared to those obtained by a reference method. The study demonstrates that the development of the allele-specific methods as pharmacogenetic tools can be simplified. (C) 2016 Elsevier B.V. All rights reserved.Tortajada-Genaro, LA.; Puchades Pla, R.; Maquieira Catala, Á. (2017). Primer design for SNP genotyping based on allele-specific amplification Application to organ transplantation pharmacogenomics. Journal of Pharmaceutical and Biomedical Analysis. 136:14-21. doi:10.1016/j.jpba.2016.12.030S142113

Chemical Surface Modifications development of Silicon Based Label Free Integrated Optical (IO) Biosensors: A Review

[EN] Increasing interest has been paid to label-free biosensors in recent years. 11 Among them, refractive index (RI) optical biosensors enable high density and the chip-12 scale integration of optical components. This makes them more appealing to help 13 develop lab-on-a-chip devices. Today, many RI integrated optical (IO) devices are made 14 using silicon-based materials. A key issue in their development is the 15 biofunctionalization of sensing surfaces because they provide a specific, sensitive 16 response to the analyte of interest. This review critically discusses the 17 biofunctionalization procedures, assay formats and characterization techniques 18 employed in setting up IO biosensors. In addition, it provides the most relevant results 19 obtained from using these devices for real sample biosensing. Finally, an overview of 20 the most promising future developments in the fields of chemical surface modification 21 and capture agent attachment for IO biosensors follows.This research has been supported by the Spanish Ministry of Science and Innovation through project CTQ2010-15943/BQU and by the Regional Valencian Government, through GVA/PROMETEO 2010/08. The authors thank Dr. Miguel Holgado, from the Universidad Politecnica de Madrid, for his helpful discussion about the classification of RI optical sensors.Bañuls Polo, MJ.; Puchades Pla, R.; Maquieira Catala, Á. (2013). Chemical Surface Modifications development of Silicon Based Label Free Integrated Optical (IO) Biosensors: A Review. Analytica Chimica Acta. 777:1-16. https://doi.org/10.1016/j.aca.2013.01.025S11677

Fluor-thiol Photocoupling Reaction for Developing High Performance Nucleic Acid (NA) Microarrays

[EN] Spatially controlled anchoring of NA probes onto microscope glass slides by a novel fluor-thiol coupling reaction is performed. By this UV-initiated reaction, covalent immobilization in very short times (30 s at 254 nm) is achieved with probe densities of up to 39.6 pmol/cm(2). Modulating the surface hydrophobicity by combining a hydrophobic silane and a hydrophilic silane allows the fabrication of tuned surfaces where the analyte approaches only the anchored probe, which notably reduces nonspecific adsorption and the background. The generated substrates have proven clear advantages for discriminating single-base-pair mismatches, and for detecting bacterial PCR products. The hybridization sensitivity achieved by these high-performance surfaces is about 1.7 pM. Finally, this anchoring reaction is demonstrated using two additional surfaces: polytetrafluoroethylene (PTFE) and polyvinylidene fluoride (PVDF) membranes. This provides a very interesting pathway for anchoring thiolated biomolecules onto surfaces with C-F motifs via a quick clean UV reaction.Financial support from INTERBOINTER (project CTQ2013-45875-R) and BIHOLOG (Project CTQ2016-75749-R), FEDER, and GVA PROMETEO II 2014/040 is acknowledged. The authors also thank Dr. Tortajada-Genaro and Dr. Ninoles Rodenes for providing the Salmonella and Campylobacter PCR-amplified products. P.J.-M. acknowledges the Spanish Ministry of Economy, Industry and Competitiveness for the public FPI grant (Project CTQ2013-45875-R) and the cofinancing by, the European Social Fund. Dr. Miguel Angel Gonzalez-Martinez, Dr. Sergio Navalon, and Dr. Patricia Concepcion from Universitat Politecnica de Valencia are acknowledged for their help in the XPS analysis.Jimenez-Meneses, P.; Bañuls Polo, M.; Puchades, R.; Maquieira Catala, A. (2018). Fluor-thiol Photocoupling Reaction for Developing High Performance Nucleic Acid (NA) Microarrays. Analytical Chemistry. 90(19):11224-11231. https://doi.org/10.1021/acs.analchem.8b00265S1122411231901

Recombinase polymerase and enzyme-linked immunosorbent assay as a DNA amplification-detection strategy for food analysis

[EN] Polymerase chain reaction in conjunction with enzyme-linked immunosorbent assay (PCR-ELISA) is a well-established technique that provides a suitable rapid, sensitive, and selective method for a broad range of applications. However, the need for precise rapid temperature cycling of PCR is an important drawback that can be overcome by employing isothermal amplification reactions such as recombinase polymerase amplification (RPA). The RPA-ELISA combination is proposed for amplification at a low, constant temperature (40 degrees C) in a short time (40 min), for the hybridisation of labelled products to specific 5'-biotinylated probes/streptavidin in coated microtiter plates at room temperature, and for detection by colorimetric immunoassay. RPA-ELISA was applied to screen common safety threats in foodstuffs, such as allergens (hazelnut, peanut, soybean, tomato, and maize), genetically modified organisms (P35S and TNOS), pathogenic bacteria (Salmonella sp. and Cronobacter sp.), and fungi (Fusarium sp.). Satisfactory sensitivity and reproducibility results were achieved for all the targets. The RPA-ELISA technique does away with thermocycling and provides a suitable sensitive, specific, and cost-effective method for routine applications, and proves particularly useful for resource-limited settings. (C) 2013 Elsevier B.V. All rights reserved.This research has been funded through Projects GV/2009/028 (Generalitat Valenciana) and CTQ/2010/15943 (MICINN). The Spanish Ministry of Education and Science provided S.S.F. with a grant for her PhD studies. The fungal cultures were supplied by B. Mora-Sala and J. Garcia-Jimenez from the Instituto Agroforestal Mediterraneo, UPV.Santiago Felipe, S.; Tortajada-Genaro, LA.; Puchades, R.; Maquieira Catala, Á. (2014). Recombinase polymerase and enzyme-linked immunosorbent assay as a DNA amplification-detection strategy for food analysis. Analytica Chimica Acta. 811:81-87. https://doi.org/10.1016/j.aca.2013.12.017818781

Actitudes y creencias machistas en Educación física hacia una profesora en prácticas

El siguiente estudio surge ante la intimidación y vulnerabilidad que sintió una profesora de Educación Física ante la mirada machista por parte del alumnado durante las primeras semanas de sus prácticas docentes en un primer curso del ciclo formativo del Grado Superior de Enseñanza y Animación Sociodeportiva en un centro de la ciudad de Valencia. Adoptando una perspectiva feminista y crítica, la profesora en cuestión desarrolló dos ciclos de Investigación-Acción (I-A) para recoger evidencias acerca de las actitudes y creencias machistas del alumnado en sus interacciones en clase, para posteriormente tratar de concienciarlos respecto al carácter discriminatorio de dichas conductas, así como de transformarlas en busca de nuevas masculinidades más igualitarias. El estudio se realizó con veintiséis estudiantes, dos profesores y una profesora de dicho curso. Durante la investigación se recogieron datos a través de dos diarios de campo de la profesora en prácticas, documentación audiovisual, seis grupos de discusión con el alumnado y un documento reflexivo del alumnado. A partir del análisis cualitativo de contenido de los datos obtenidos, los resultados presentan evidencias de cómo las actitudes machistas en las aulas no sólo se dan entre el alumnado, sino en la relación del alumnado con el profesorado. Estas actitudes adquieren más relevancia cuando la relación es con una profesora en prácticas. El texto abre nuevas líneas de investigación dentro de los estudios feministas, en la medida en que propone seguir explorando cómo afectan las actitudes machistas del alumnado a la tarea de las docentes y en concreto de las docentes en prácticas. The following study arises from the intimidation and vulnerability that a Physical Education (PE) teacher felt in the face of the students' macho gaze during the first weeks of her teaching practices in the 1st year of the Advanced Vocational Training Course on Socio-sports Teaching and Animation of a center located in Valencia. Adopting a feminist and critical perspective, the teacher in question developed two cycles of Action-Research (A-R) to collect evidence about the macho attitudes of the students in their interactions in class, to later try to make them aware of the discriminatory nature of said attitudes, as well as transforming them into new and more egalitarian masculinities. The study was carried out with twenty-six students, two male teachers, one female teacher and a female trainee teacher. During the investigation, data were collected by means of two field diaries of the teacher in practice, audiovisual documentation, six focus groups with the students and students' reflection documents. After performing the qualitative content analysis, the text presents evidence of how sexist attitudes in the classroom not only occur among students, but also in the relationship between students and teachers. These attitudes become more relevant when it comes to trainee teachers. The study opens new lines of research within feminist studies, to the extent that it proposes to continue exploring how the male chauvinist attitudes of students affect teachers' work, specifically trainee teachers' work